Journal: Frontiers in Immunology

Article Title: Synergetic role of TRPV4 inhibitor and mechanical loading on reducing inflammation

doi: 10.3389/fimmu.2024.1456042

Figure Lengend Snippet: Confocal microscopy images of the cell nucleus stained with DAPI, F-actin with phalloidin, pro-inflammatory (CD80), and anti-inflammatory marker (CD206). Representative of 5 images per group (Scale bar =15 μm).

Article Snippet: Overnight incubation at 4°C was conducted with mouse monoclonal CD80 antibody (1:100, Invitrogen, TA501575) and recombinant rabbit CD206 antibody (1:100, ThermoFisher, MA1-35936), selected as pro-inflammatory and anti-inflammatory macrophage-related surface markers, respectively.

Techniques: Confocal Microscopy, Staining, Marker

Journal: International Journal of Molecular Sciences

Article Title: Drugs That Induce Gingival Overgrowth Drive the Pro-Inflammatory Polarization of Macrophages In Vitro

doi: 10.3390/ijms252111441

Figure Lengend Snippet: Immunofluorescence staining of the adhered macrophages after differentiation. Magnification 20X. ( a , b ) M0 macrophages negative for CD80 and CD163, respectively; ( c ) M1 macrophages positive for CD80 (red); ( d ) M1 macrophages negative for CD163; ( e ) M2 macrophages positive for CD163 (green); ( f ) M2 macrophages negative for CD80. Nuclei were stained with DAPI (blue).

Article Snippet: Cells were then incubated overnight at 4 °C with mouse anti-human CD80 monoclonal antibody (Thermo Fischer Scientific, Waltham, MA, USA) and rabbit anti-human CD163 monoclonal antibody (Thermo Fischer Scientific, Waltham, MA, USA).

Techniques: Immunofluorescence, Staining